Qiaquick gel extraction kit 250 pdf

Qiaquick gel extraction kit 250 from qiagen selectscience. Qiagen qiaquick pcr purification kit, 250 rxns, 10g binding capacity, 30l elution volume, tube format, manual processing, silica technology, 100 bp to 10 kb fragment. This kit can also be used for dna cleanup from enzymatic reactions see page 8. The innovative qiacube uses advanced technology to.

Add 3 volumes of buffer qg to 1 volume of gel i used the back of 200ul tips for gel excision and assumed the weight was about 150mg and therefore used 450ul of buffer qg 3. For dna fragments 8 kb, following the incubation step, add one additional volume equal to that of the gel slice of. Gel extraction protocol qiaquick gel extraction kit. Qiagen qiaquick gel extraction kit, 250 rxns, 30 to 50l elution volume, 10g binding capacity, dna sample, tube format, silica technology, manual processing, 70 bp to 10 kb fragment, fast and convenient procedure, for gel extraction cleanup of up to 10. The qiaquick gel extraction kit enables removal of nucleotides, enzymes, salts, agarose, ethidium bromide, and other impurities from samples, ensuring up to 80% recovery of dna see figure high recoveries from gels. Jenelle meyer 62007 university of wisconsinmadison. Add 3 volumes buffer qg to 1 volume gel 100 mg gel 100. Qiaquick pcr purification kit protocols 18 using a microcentrifuge 18 using a vacuum manifold 19 qiaquick nucleotide removal kit protocol 21 qiaquick gel extraction kit protocols 23 using a microcentrifuge 23 using a vacuum manifold 25 troubleshooting guide 28 appendix. Qiavac vacuum manifolds 30 handling guidelines for qiavac 6 31 qiavac 24 32 ordering information 33 qiagen companies and distributors 35 qiaquick spin handbook 072002 3.

Invitrogen purelink pcr purification kit 250 preps. Add 3 volumes of adb to each volume of agarose excised from the gel e. Why is the concentration of dna very low after a gel extraction. Qiaquick pcr purification kit protocol using a microcentrifuge this protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8.

This products resides on a fisher scientific gsa or va contract. Qiaquick pcr purification kit 250 from qiagen sample to. Qiaquick gel extraction kit protocol using a microcentrifuge chen. The minelute pcr purification kit and minelute gel extraction kit can be fully automated on the qiacube. For purification of up to 10 micro g pcr products, 100 bp to 10 kb. Collection tubes 2 ml 50 250 loading dye 110 l 550 l handbook 1 1 minelutegel extraction kits 50 250 catalog no. Qiavac vacuum manifolds 30 handling guidelines for qiavac 24 31 qiavac 6 32. Can qiaquick gel extraction kit purified dna be used for. The qiaquick gel extraction kit provides spin columns, buffers, and collection tubes for silicamembranebased purification of dna fragments from gels up to 400 mg slices or enzymatic reactions. Incubate at 50c for 10 min or until the gel slice has completely dissolved.

Qiagen qiaquick gel extraction kit, 250 rxns, 30 to 50l elution volume, 10g binding capacity, dna sample, tube format, silica technology, manual processing, 70 bp to 10 kb fragment, fast and convenient procedure, for gel extractioncleanup of up to 10micro g. Qiagen qiaquick pcr purification kit, 250 rxns, 10g binding capacity, 30l elution volume, tube format, manual processing, silica technology, 100 bp to 10 kb fragment, 40mers fragments removed, ideal for sequencing, microarray analysis, ligation and transformation, restriction. Regardless of 260230 or 260280, it is always optimal to get at least 1. This kit can also be used to purify dna from polyacrylamide gels. Gel extraction protocol qiaquick gel extraction kit protocol 1. The purelink quick gel extraction kit allows you to rapidly and efficiently purify dna fragments from tae or tbe agarose gels of various percentages. Related products include the qiaquick nucleotide removal kit and qiaquick gel extraction kit. Recoveries of dna fragments in the size range between removed and recovered are not defined. Using a microcentrifuge or vacuum manifold, dna ranging from 70 bp to 10 kb is purified from 124 samples. Qiaquick nucleotide removal kit protocol 21 qiaquick gel extraction kit protocols 23 using a microcentrifuge 23 using a vacuum manifold 25 troubleshooting guide 28 appendix. Automated dna cleanup the qiaquick pcr purification kit and qiaquick gel extraction kit can be fully automated on the. These are available online in convenient and compact pdf format.

For cleanup of other enzymatic reactions, follow the protocol as described for pcr samples or use the minelute reaction cleanup kit. If you are viewing this page as a nonregistered user, the prices displayed is list price. The genelute gel extraction kit is designed for the rapid purification of linear and plasmid dna fragments from standard or lowmelting agarose gels. From solutions from gels qiaquick qiaquick pcr nucleotide qiaquick gel qiaquick gel purification kit removal kit extraction kit extraction kit alkaline phosphatase yes yes yes yes cdna synthesis yes no no yes. Plasmid dna purification genomic dna purification cat. Apgx4 apgx50 apgx 250 kit size 4 preps 50 preps 250 preps miniprep column 4 50 250 2 ml microfuge tube 4 50 250 1. Qiagen qiaquick gel extraction kit, 250 rxns, 30 to 50l elution volume, 10g binding capacity, dna sample, tube format, silica technology, manual processing, 70 bp to 10 kb fragment, fast and convenient procedure, for gel extractioncleanup of up to 10. Qiaquick gel extraction kit protocol using a microcentrifuge. Poor yield with gel extraction kit for about the umpteenth time now, i have gotten poor yield isolating my cut fragment from a 1% agarose gel. This protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. Qiaquick gel extraction kit the qiaquick gel extraction kit cat. This protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. I put in 1ug and i am getting back about 10nul but, my 260280s are jumping all over the place, as is my concentrations. Incubate at 3755 c for 510 minutes until the gel slice is completely dissolved2.

Banerjee lab ucla molecular, cell and developmental biology. Gel extraction protocol qiaquick gel extraction kit protocol. Dna ranging from 70 bp to 10 kb is purified using a simple and fast bindwashelute procedure and an elution volume of 3050. A260230 readings post gel extraction general lab techniques. For dna cleanup from enzymatic reactions using this protocol, add 3. Qiaquick pcr purification kit 250 from qiagen selectscience. Axyprep dna gel extraction kit for the rapid purification of dna fragments from agarose gels kit contents, storage and stability cat. I used the qiaquick gel extraction kit and followed the protocol supplied with the kit. Dna can be extracted and purified from agarose gels with different melting points in 30 minutes using purelink silica membranebased quick gel extrac. Qiaquick gel extraction kit protocol syracuse university.

Qiaquick gel extraction kit protocols 23 using a microcentrifuge 23 using a vacuum manifold 25 troubleshooting guide 28 appendix. Kit contents qiaquick pcr purification kits 50 250 catalog no. Brand thermo scientific qiagen machereynagel promega invitrogen kit and no. Up to 400 mg agarose can be processed per spin column. For departmental related matters, please visit the department of molecular, cell, and developmental biology website. Genelute gel extraction kit 70 purifications sigmaaldrich. This kit is specially designed for fast and easy isolation of smaller dna fragments 70 bp 4 kb from pcr reactions, agarose gels, or enzymatic reactions with an elution volume of only 10 l. For gel extractioncleanup of up to 10 micro g dna 70 bp to 10 kb from enzymatic reactions. These are available online in convenient and compact pdf format at. The maximum amount of gel per spin column is 400 mg.